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2 edition of Ultraviolet light mutagenesis in Myxococcus Xanthus. found in the catalog.

Ultraviolet light mutagenesis in Myxococcus Xanthus.

Richard Bruce Brown

Ultraviolet light mutagenesis in Myxococcus Xanthus.

by Richard Bruce Brown

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Published by University of East Anglia in Norwich .
Written in English


Edition Notes

Thesis (Ph.D.), University of East Anglia, School of Biological Science, 1985.

ID Numbers
Open LibraryOL13846949M

Effects of site-directed mutagenesis of mglA on motility and swarming of Myxococcus xanthus Sarah A Fremgen1, Neal S Burke1,2, Patricia L Hartzell1* Abstract Background: The mglA gene from the bacterium Myxococcus xanthus encodes a 22kDa protein related to Cited by: Fruiting body development of Myxococcus xanthus is induced when amino acid depletion forces the cells to decide whether to grow or develop. Central to the decision is the stringent response, which shares many features with that of Escherichia coli (for review, see Cashel et al. ).When ribosomes stall because of lack of charged tRNA, the ribosome-associated protein RelA synthesizes.

xanthus. It has been shown to contain two copies of the recA gene, only one of which (recA2) is DNA damage-inducible (Norioka et al., ). Likewise, the existence of cell reactivation of UV-irradiated phages as well as of UV-mediated mutagenesis have been also reported in M. xanthus (Herdrich and Grimm, ; McCann and Clarke, ).Cited by:   Among myxobacteria, Myxococcus xanthus has emerged as a model organism to understand fruiting body formation, and most Myxococcus generate simple haystack-shaped fruiting bodies. s is a Gram-negative rod-shaped bacterium. During fruiting body development roughly , individual cells aggregate to form the fruiting body.

Sensing Prey Signal Transduction. Myxococcus xanthus uses a frz signal transduction system to control its directed movement towards frz system is similar to the Che chemotaxis system in E. coli, and frz gene mutants exhibit similar characteristics to well-known che gene mutants in the E. coli system. In M. xanthus, however, chemotactic activity is controlled by reversing direction.   One of the key limitations to genetic manipulation in myxobacteria is that the cells grow in clumps in liquid. A salt-tolerant strain HW-1 of Myxococcus fulvus was treated with UV irradiation and produced a completely dispersedly growing mutant UV There were no significant differences between the parent HW-1 and the mutant UV in terms of salt-tolerant growth. The mutant UV .


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Ultraviolet light mutagenesis in Myxococcus Xanthus by Richard Bruce Brown Download PDF EPUB FB2

In the ultraviolet (UV)-mutable bacterium, Myxococcus xanthus, dose response curves for the induction of rifampicin-resistant (Rifr) mutants were compared with dose response curves for Weigle(W)-reactivation of the UV-irradiated phage Mx4 at a phage survival of 5 X 10(-6).Cited by: 1.

In the ultraviolet (UV)-mutable bacterium, Myxococcus xanthus, dose response curves for the induction of rifampicin-resistant (Rifr) mutants were compared with dose response curves for Weigle(W)-reactivation of the UV-irradiated phage Mx4 at a phage survival of 5x10− by: 1.

Ultraviolet light mutagenesis in Myxococcus xanthus Author: Brown, R. ISNI: Awarding Body: University of East Anglia Current Institution: University of East Anglia Date of Award: Availability of Full Text: Full text unavailable from EThOS.

Plasmid R46 was successfully transferred from Escherichia coli K into Myxococcus xanthus strain MD-1 but not into M. xanthus strain XK. Plasmid R w The effects of these plasmids on survival of M.

xanthus after ultraviolet (UV) nm irradiation, the ability of M. xanthus to reactivate irradiated myxophages, and Weigle reactivation of UV-irradiated myxophages by M. xanthus were Cited by: 1.

Targeted Mutagenesis of s54 Activator Proteins in Myxococcus xanthus LISA GORSKI AND DALE KAISER* Department of Biochemistry and Department of Developmental Biology, Stanford University School of Medicine, Stanford, California Received 19 December /Accepted 9 September Cited by: Myxococcus xanthus mutants defective in myxospore germination have been isolated both by a selective and by a non-selective method after UV or Tn5-lac-induced mutagenesis.

Bacteriol.€, (19) S S Wu and D Kaiser € gene. counterselection with the Bacillus subtilis sacB Myxococcus xanthus generated by. In the ultraviolet (UV)-mutable bacterium, Myxococcus xanthus, dose response curves for the induction of rifampicin-resistant (Rifr) mutants were compared with.

The Myxococcus xanthus pilQ (sglA) gene encodes a secretin homolog required for type IV pilus biogenesis, social motility and development. Bacteriol.24– 33 ().Cited by: UV mutagenesis in Escherichia coli K Cell survival and mutation frequency of the chromosomal genes lacZ, rpoB, ompF, and ampA KEVIN LIN AND ALICE WANG Department of Microbiology and Immunology, UBC UV-induced mutation frequency and cell survivability was examined in Escherichia coli K File Size: KB.

Myxococcus xanthus is a predatory bacterium and a model system for social behaviour in bacteria. Myx. xanthus forms thin biofilms, where cells work together to colonize new territory, invade prey colonies and lyse prey cells.

Prey-cell lysis occurs at close proximity, and utilizes antibiotics such as myxovirescin, hydrolytic enzymes such as the protease MepA and extracellular outer-membrane Cited by: Original producer, UV mutagenesis and protoplast fusion 1 Myxococcus xanthus Deltaproteobacteria Gram-negative μg/L 2 Myxococcus xanthus Deltaproteobacteria Gram-negative 23 mg/L Fed-batch process 3 Myxococcus xanthus Deltaproteobacteria Gram-negative ~ μg/L transposition 4.

Ultraviolet light mutagenesis in Myxococcus xanthus Author: Brown, R. Awarding Body: University of East Anglia Current Institution: University of East Anglia Date of Award: Availability of Full Text.

The myxobacterium Myxococcus xanthus is a predatory member of the soil microfauna, able to consume bacteria (Gram-negative, Gram-positive), archaea, and fungi. Many potential prey of M. xanthus communicate amongst themselves using acyl homoserine lactones (AHLs) as quorum signals. xanthus cannot itself produce AHLs, but could potentially benefit by responding to exogenous Cited by: SUMMARY: Myxococcus xanthus mutants defective in myxospore germination have been isolated both by a selective and by a non-selective method after UV or Tn 5-lac-induced ability of these mutants to germinate in germinant solutions other than those used for their isolation has been tested.

Six of seven mutants isolated behaved as germination-defective in all by: 2. The intercellular C-signal has a fundamental role in fruiting body morphogenesis in Myxococcus xanthus. In this chapter, our current understanding of how the C-signal acts at the molecular level to induce and coordinate events that are separated in time and space is by: About this Book In accordance with its predecessor, the completely revised and expanded Second Edition of Modern Microbial Genetics focuses on how bacteria and bacteriophage arrange and rearrange their genetic material through mutation, evolution, and genetic exchange to take optimal advantage of their environment.

One such behavior is kin recognition, in which cells identify siblings via their polymorphic TraA cell surface receptor, to transiently fuse outer membranes and exchange their contents. In addition, outer membrane exchange (OME) regulates behaviors, such as inhibition of wild-type Myxococcus xanthus (DK) from swarming.

Here we monitored the fate of motile cells and surprisingly found Cited by: Myxococcus xanthus: Taxonomy navigation › Myxococcus. All lower taxonomy nodes (4) Common name i-Synonym i- Other names i ›ATCC ›CCUG ›CIP ›DSM ›IFO More» «Less: Rank i: SPECIES: Lineage i.

Myxococcus xanthus DK has two RecA genes, recA1 (MXAN_) and recA2 (MXAN_), with unknown functional differentiation. Herein, we showed that both recA genes were induced by ultraviolet (UV) irradiation but that the induction of recA1 was more delayed than that of recA2.

Deletion of recA1 did not affect the growth but significantly decreased the UV-radiation survival, homologous Author: Duo-Hong Sheng, Yi-Xue Wang, Miao Qiu, Jin-Yi Zhao, Xin-Jing Yue, Yue-Zhong Li. Myxococcus xanthus is a Gram-negative bacterium commonly found in soil that shows a social behavior: the cells move in a coordinated fashion and form a biofilm.

M. xanthus cells do not have flagella, but rather have type IV pili and glide on solid r, the pilus system may work by the same physics principle as the flagellum system; both have Mot or Mot homologs powered by .This chapter focuses primarily on transcriptional regulation of developmental genes.

While the identification of developmentally regulated Myxococcus xanthus genes continues, now on a comprehensive genome-wide scale with the use of DNA microarray expression profiling, an understanding of the cis-acting DNA elements and trans-acting proteins (RNA polymerase [RNAP] Cited by:   Abstract.

Myxococcus xanthus BtkB is composed of an N-terminal periplasmic domain and a C-terminal cytoplasmic tyrosine kinase domain. The C-terminal cytoplasmic domain of BtkB was autophosphorylated in the presence of [γ P]ATP and MgCl 2, and the autophosphorylated BtkB was detected with antiphosphotyrosine antibody, suggesting that BtkB is a bacterial tyrosine (BY) by: 9.